The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Simplified workflow: Add cellular imaging assays in a small footprint. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Find our technical support information along with contact details. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Increased fluorescence performance. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The assay window spanned four orders of magnitude. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. Multimode Readers Comparison Chart. The CellTiter-Glo assay from Promega uses luciferase. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. china@moldev. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 0´ 104 8. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. 2. Lower limits of detection for ATP determined using the ATPlite . The SoftMax Pro Software quickly. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. We would like to show you a description here but the site won’t allow us. SpectraMax i3x, onboard luminescence 121. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. . 5. Figure 4. When the user applies a set temperature of 37°C, the top is heated to 37. Price from $9. , 2021). Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Eliminate time-dependent substrate addition steps. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The treatments were. 58% for the detection module and 2. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 11. ZERO BIAS - scores, article reviews, protocol conditions and more. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Cet appareil ISM est confomre à la norme NMB-001 du Canada. VAL014, right table) assays on the SpectraMax i3x reader are shown below. Download PDF. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Description 1. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Category 2 and 3 microplate readers use gradient heating as well. Contact our support team with your questions regarding Molecular Devices products and applications. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. 0. 0. Published Dec 16, 2020 | Updated Oct 10, 2023. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. A: percent GFP-positive cells calculated using the classification feature in the software. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. 흡광 (Absorbance) 리더기. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 660-665 Eskdale Road. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Patented user-exchangeable cartridge design expands the system's detection capability making it. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. The size and fluorescence intensity were tested for 48 h. SpectraMax i3x Multi-Mode Detection Platform User Guide. Analysis. Multimode Reader Comparison Chart. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. com. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. Description 1. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. 5 µL anti-cAMP-Cryptate. 9 x 10-15. 6 in. 6% for the on-board optics (monochromators). Modularity does provide some measure of forward vision into upcoming or future experimental needs. 58% for the detection module and 2. QBT Fatty Acid Transporter Assay. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. In addition, Image J was used to quantify the. Native or oxidized AN (40 μM) and FN (0. Figure 1. HTRF IP standard curve. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. ) deep, preferably with access on all sides. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. *On the SpectraMax i3x reader only. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. applications, including dual luciferase and ATP assays. 吸収度マイクロプレートリーダー SpectraMax® Mini. 2. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Description 1. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. We would like to show you a description here but the site won’t allow us. Molecular Devicesの全商品を見る. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Remove the transport bracket and store the retaining screws in the bag that held the 3. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. infoboxeu@moldev. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Fluorescence Reader. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. Find regional support. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The chamber. Unlock your i3/i3x instrument after shipment. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 305 reference level saturation. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. HTRF-compatible SpectraMax Multi-Mode Readers. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. ZERO BIAS - scores, article reviews, protocol conditions and more. The turbidity. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). Examples: fiPress carefully to engage,fl fiDo not press down. How to properly unlock iD series instrument. Molecular Devices are known for both the reliability and the wide uses of their products. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. SpectraMax i3x Multi-Mode Detection Platform User Guide. Winnersh Triangle. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. 4 mg mL −1; 1. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. ) wide and 65 cm (25. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Spectramax I3x, supplied by Danaher Inc, used in various techniques. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. User-upgradeable detection modules allow you to expand the capabilities of your microp. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Materials and methodsWe would like to show you a description here but the site won’t allow us. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Increased fluorescence performance. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The drawing shows a standard microplate of 15 mm height covered with a lid. 635. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 3: The cuvette chamber. Pick the best speed, sensitivity, and price for your needs. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Sensitive TRF detection of two simple, mix-and-measure assay workflows. . For wavelengths in the UV range above 220 nm,. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. MTT was purchased from Sigma-Aldrich (St. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. SpectraMax® iD3/iD5. Increased fluorescence performance. 吸収度マイクロプレートリーダー SpectraMax® Mini. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. Nucleic Acid and Protein Quantitation. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Register to download your free eBook today. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 200-800 nm using Spectra Max i3X microplate reader. , 2011). Get a front row view of your cells. ). The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Home > Search Results > Danaher Inc > spectramax i3x. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Bioz Stars score: 98/100, based on 1 PubMed citations. ATP (“flash” luminescence using . Prepare your iD series instrument for shipment. All test measurements and calculations are handlediD5. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 3) At the bottom of the strip, click on "Show. 4. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. com Check our website for a current listing of worldwide distributors. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. 1step assay with SpectraMax microplate readers. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Download PDF. Prime function 20 amol. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Thetypeof. 2 (Molecular Devices, San Jose, CA). 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Controls containing all reaction components with either DI . Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. Find regional support. +44 118 944 8000. See SpectraMax® Microplate Reader User Manual Download Page. D 1 1. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Use the 3. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Budget-friendly multi-mode microplate reader . SpectraMax i3x and Paradigm readers require the FP-FLUO. 2. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Nuclear Receptor. 7°C and the bottom to 36. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The corrected absorbance is within 5% of the. , 2011). User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. 312 gain calibration failed. Anne T. The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Bioz Stars score: 98/100, based on 1 PubMed citations. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. . Methods. i3. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Prismatic colour and motion for everyone. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. 2. com. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. The plate was shaken and incubated in the dark for 10 minutes. The device can be upgraded to include capabilities for Western blotting, imaging and injection. Explore a wealth of applications with the future-ready SpectraMax i3x. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. each new lot of plates, to ensure that the center of each well is read. 2 in. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. applications, including dual luciferase and ATP assays. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. ) deep, preferably with access on all sides. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Data Analysis. The SpectraMax i3x reader provides the. Published Dec 16, 2020 | Updated Dec 16, 2020. To detect physical stability, Pdots were treated with water and incubated with plants. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. com. . Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Manufacturers try to design their instruments so they can be. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Spectramax I3x, supplied by Danaher Inc, used in various techniques. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . The corrected absorbance is within 5% of the value. Assay setup for a 384-well low-volume plate. The dynamic range of the assay is about 3. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Danaher Inc spectramax i3x. i3x. i3. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Shake intensity. 4 / 5. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. detection cartridge LUM96: 59. The dual auto injector module. Data Analysis. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. 315 can't find zero order. g. The chamber. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. 4 μM), prepared as described in section 2. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. 00800 665 32860. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. Find regional support. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. The SpectraMax i3x reader provides theAbsorption was measured at 490 nm by utilizing a Spectra max i3x Multimode microplate reader (Molecular Devices). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The plate was shaken and incubated in the dark for 10 minutes. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. The assay window spanned four orders of magnitude. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 3: The cuvette chamber. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. SoftMax Pro GxP Compliance Software. This way you’ll save time on finding the necessary info. Table 1. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. This is not due to a temperature-dependent alteration in cellular autofluorescence,.